N,N’ -methylene diacrylamide (MBAm or MBAA) is a crosslinking agent used in the formation of polymers such as polyacrylamide. Its molecular formula is C7H10N2O2, CAS: 110-26-9, properties: white crystalline powder, soluble in water, also soluble in ethanol, acetone and other organic solvents. Diacrylamide is a compound of polyacrylamide gel (for SDS-PAGE) that can be used in biochemistry. Diacrylamide polymerizes with acrylamide and is able to create cross-links between polyacrylamide chains, thus forming a polyacrylamide network rather than unconnected linear polyacrylamide chains.
Crosslinking agent
In chemistry and biology, crosslinking is a bond that connects one polymer chain to another. These links may take the form of covalent or ionic bonds, and the polymer may be synthetic or natural (e.g. protein).
In polymer chemistry, “crosslinking” usually refers to the use of crosslinking to promote changes in the physical properties of the polymer.
When “crosslinking” is used in the field of biology, it refers to the use of probes to link proteins together to examine protein-protein interactions and other innovative cross-linking methods.
Although the term is used to refer to “linking of polymer chains” in both sciences, the degree of crosslinking and the specificity of the crosslinking agent vary widely. As with all sciences, there is overlap, and the following description is a starting point for understanding these nuances.
Polyacrylamide gel electrophoresis
Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensics, genetics, molecular biology, and biotechnology for the separation of biological macromolecules (usually proteins or nucleic acids) based on their electrophoretic mobility. Electrophoretic mobility is a function of molecular length, conformation, and charge. Polyacrylamide gel electrophoresis is a powerful tool for analyzing RNA samples. When the polyacrylamide gel is denatured after electrophoresis, it provides information about the composition of the RNA type sample.
Other uses of N,N’ -methylene diacrylamide
N,N’ -methylene diacrylamide as a chemical reagent has a wide range of applications, it is oilfield fracturing fluid, superabsorbent resin, water blocking agent, concrete additives, alcohol soluble sexy light nylon resin, water treatment flocculant synthesis of an important additive, it is also a good water absorption agent and water retention agent, used in the manufacture of superabsorbent resin and soil improvement, Also used for photography, printing, plate making, etc.
Post time: Feb-15-2023